Gomutra Therapy for Cancer

Recent Research on the Anti-Cancer Activity of Cow Urine 

Parnayavani (Plectranthus amboinicus) with Gomutra Ark in vivo and in vitro- 

• Mani, Vino & V, Nithya. (2021). Evaluation of the anticancer activity of Plectranthus amboinicus with Cow ark against CCl4 Induced Hepatocellular carcinoma – In Vivo. Annals of the Romanian Society for Cell Biology. 25. 19406-19414. Background: Natural origin alternatives to synthetic drugs are beneficial in curing human lung and liver ailments. The present study aims to evaluate in vivo the Anticancer effects of Cow urine distillate with Plectranthus Amboinicus (CPA), on the hematological implications, using CCL4 induced Wister albino rats model. Methods: The indigenous cow, Bos indicus urine was distilled and characterized to know the latent compounds present in CUD, enhanced with Plectranthus Amboinicus, and its functional groups by employing HPLC and FTIR techniques. Further, In vivo, anti-cancer activity was determined against HCC by evaluating HCC-induced rats’ life span, and hematological profile, such as RBC, WBC, Hb content, neutrophils, eosinophils and pellets. Results: Cow urine with Plectranthus Amboinicus treatment significantly reduced viable cancer cell count, and increased the life span of HCC-inducing rats. Hematological profile retrieved to normal position in Cow urine distillate treated groups when compared with normal rat groups.
• V, Nithya & Thavasuraj S, Dr. (2020). Effect of Indigenous cow ark with Plectranthus amboinicus for anticancer activity – In vitro. GEDRAG & ORGANISATIE REVIEW. 33. 10.37896/GOR33.03/461. Background / Aim: The distillate Cow urine (cow ark) enhanced with medicinal plants, a vital role in the treatment of many diseases. It has been aimed at in vitro antioxidant and anticancer activity of distilled cow urine with Plectranthus amboinicus against cervical cell line. Materials and method: The cow urine was photoactivated (cow ark) and extracted with Plectranthus amboinicus. The antioxidant activity of cow ark with Plectranthus amboinicus (CPA) using DPPH radical scavenging assay and reducing power assay. The growth of cell viability and cytotoxicity were observed by using MTT assay. Results: From these results, the CPA has good antioxidant potential to reduce oxidative stress. The growth of the cancer line viability has been decreased with the concentration of 100 mg/ ml showed 67.26 % treated with CPA. The cytotoxicity of cancer line inhibition of 74.139 % against the CPA treated. In this method, the CPA showed an IC50 value of 56.23 against the cervical cancer cell line. Conclusion: It is concluded that the cow ark Plectranthus amboinicus has tremendous potential for cervical cancer disease.
• Phenolics were the major compounds that could be responsible for anticancer activity. Dietary and non-dietary phenols have been well-reported to possess anticancer activity and modulate the aforementioned mechanisms. Several studies showed evidence regarding the effect of phenols on the regulatory proteins of signaling pathways that ultimately leads to apoptosis (Ramos 2008, Mathan et al., 2016).
• Jain, N. & Gupta, V. & Garg, Rajesh & Silawat, N. (2010). Efficacy of cow urine therapy on various cancer patients in Mandsaur District, India – A survey. International Journal of Green Pharmacy. 4. 10.4103/0973-8258.62163. Evaluation of cow urine therapy on cancer patients in 8 8-day camps at Mandsaur district was carried out. The object of this survey was to evaluate the efficacy of cow urine therapy on various cancer patients who were reported across different states of India. A total of 68 cancer patients were reported during the survey from 8 April 2007 to 15 April 2007. A questionnaire was developed to assess the efficacy of cow urine therapy. During the survey, out of 68 patients, 7.35 % of patients withdrew themselves from the treatment, and 63 (92.64 %) patients continued the therapy. There was a high proportion (30.87 %) of throat cancer and the other prevalent cancer was breast cancer (14.70 %) followed by cervix and uterine cancer (5.88 %), buccal cavity cancer, and sinus (4.41%) lung cancer, lymphoma and bone cancer (2.94 %), both throat and buccal (5.88 %) and other cancer (8.82 %), respectively. The symptoms (pain, inflammation, burning sensation, difficulty in swallowing, irritation, etc.) of cancer patients were categorized into severe, moderate, and mild categories, respectively. An intensive study of the data of cancer patients revealed that the degree of severe, moderate, and mild symptoms was 82.16 %, 15.8 %, and 1.58 % on the first day and 7.9 %, 55.3 % and 36.34 % on the eighth day, respectively. It was evaluated that patients who were receiving cow urine therapy for 2-3 months were most benefited. Hence, this traditional therapy may be a boon to cancer patients.
• Kannappan Mohanvel, Sucharitha & Rajasekharan, Satish Kumar & Kandhari, Trishna & Doss, Balaji & Thambidurai, Yuvarani. (2017). Cow urine distillate as a bioenhancer for antimicrobial & antiproliferative activity and redistilled cow urine distillate as an anticlastogen agent. Asian Journal of Pharmaceutical and Clinical Research. 10. 273. 10. 22159/ ajpcr. 2017. v10i10. 18879. Objective: The objective of this study was to prove that cow urine distillate (CUD) is a bioenhancer for antimicrobial activity and antiproliferative activity, redistilled CUD (RCUD) as an anticlastogen agent. Methods: The antimicrobial activity of rifampicin with CUD at different concentrations was determined against pathogenic Escherichia coli by well puncture method. The penicillin and ciprofloxacin in combination with CUD at different/increasing concentrations against pathogenic E. coli culture were also determined by the disc diffusion method. Sulforaphane (ACA) as an anticancer agent was extracted from cruciferous vegetables and purified by high-performance liquid chromatography. The Breast cancer cell lines (MCF- 7) were treated with anticancer agents along with CUD in increasing concentrations. The anticlastogenic activity of RCUD in human peripheral lymphocytes was tested with clastogens such as manganese dioxide and hexavalent chromium. Results: CUD was shown to enhance the antimicrobial activity of rifampicin with 20 μl concentration by well puncture method; and penicillin with increasing concentration of up to 80 μl and ciprofloxacin up to 80 μl, respectively, by disc diffusion method. The rate of degeneration of breast cancer cell lines (MCF-7) was increased with increasing concentration of CUD. Clastogen (MnO2) of 10 μl with 200 μl of RCUD showed effective anticlastogenic activity in agarose gel electrophoresis as the activity of clastogen decreased with increasing concentration of RCUD.Conclusion: CUD acts as a bioenhancer to increase antimicrobial and antiproliferative activity. RCUD showed a high level of anticlastogenic activity toward clastogen. Thus, cow urine is found to have special properties that can be used in combination with different therapeutic agents to cure several diseases such as tuberculosis, leprosy, and cancer. Further in vivo and clinical studies are required to confirm its therapeutic efficacy.
• D’souza, P.F. & Ashoka, S. & Rajan, M.S. & Shabaraya, A. R. (2013). Anti-tumor activity of mercaptopurine in combination with trikatu and gomutra on 20- methylcholantrene induced carcinogenesis. Journal of Applied Pharmaceutical Science. 3. 20- 24. 10. 7324/ JAPS. 2013. 3804. Cancer is one of the most life-threatening diseases and serious health problems in both developing and developed countries. Many synthetic and chemotherapeutic agents used in cancer therapy have low bioavailability and involve the risk of life-threatening host toxicity. Modern researchers are increasingly showing interest in the improvement of the bioavailability of a large number of drugs by the addition of various herbs with bioenhancing properties. In oral drug delivery systems, the co-administration of therapeutic agents with natural compounds possessing absorption-improving activities has also garnered great interest. Hence the present study was conducted to evaluate the anti-tumor activity of Mercaptopurine in combination with Trikatu and Gomutra. 20- Methylcholanthrene, a Polycyclic aromatic hydrocarbon was used to induce tumors in albino mice. Hematological and endogenous antioxidant parameters were evaluated in the study. Individual treatment with Mercaptopurine (5 mg/ kg) and trikatu (100 mg/ kg) significantly restored the altered hematological and antioxidant parameters to normal values. Even Mercaptopurine (2.5 mg/ kg) at its sub-therapeutic dose showed equivalent effects as that of the therapeutic dose of Mercaptopurine (5mg/ kg) when it was co-administered along with trikatu compared to the positive tumor control group.
• Joshi, Ankita & Bankoti, Kamakshi & Bisht, Tanu & Chauhan, Ramswaroop. (2012). Immunomodulatory Effect of Gir Cow Urine Distillate in Rabbits. Journal of Immunology and Immunopathology. 14. 57. 10.5958/j.0972-0561.14.1.009. In the present investigation, the immunomodulatory effect of distilled Gir cow urine was studied in rabbits for hematological parameters. The study revealed that the values of total leucocyte count (TLC), absolute lymphocyte count (ALC), and absolute neutrophil count (ANC) were significantly increased in Group II and the rabbits were given Gir cow urine distillate alone and Gir cow urine distillate with citric acid, respectively. By comparing the studies on the 60th day, there was an increase in total leucocyte count (TLC), absolute lymphocyte count (ALC), and absolute neutrophil count (ANC) levels in group II as 29%, 24.50% and 32%, respectively. In group III, an increase in total leucocyte count (TLC), absolute lymphocyte count (ALC) and absolute neutrophil count (ANC) levels were 49%, 41%, and 49%, respectively. This indicates the immunomodulatory effect of Gir cow urine alone and with citric acid.
• Akula, Annapurna & Vishala, Chandi & Pitchaiah, Gummalla. (2017). IMMUNOMODULATORY ACTIVITY OF NUTRACEUTICAL FORMULATION AND ITS POTENTIATION BY SELF-FORTIFICATION AND COW URINE DISTILLATE FORTIFICATION METHODS. International Journal of Pharmacy and Pharmaceutical Sciences. 9. 15. 10.22159/ijpps.2017v9i8.13716. Objective: This study prepared, and evaluated the immunomodulatory activity of nutraceutical formulation and studied the effect of self-mortification and cow urine distillate fortification methods on the immunomodulatory potential of nutraceutical formulation.Methods: Three types of nutraceutical formulations i.e. Nutraceutical formulation (NF), self-fortified nutraceutical formulation (SFNF), and self-fortified nutraceutical formulation fortified with cow urine distillate (SFNECUD) were prepared using fine powders of amla, apple, garlic, onion, wheat grass, papaya, turmeric, and cow urine distillate by different methods. The immunomodulatory activity of nutraceutical formulations at a dose of 500 mg/kg was assessed by various immune function parameters like cell-mediated immunity (neutrophil adhesion, delayed-type hypersensitivity (DTH) response and cyclophosphamide-induced neutropenia), humoral immunity (serum immunoglobulins level and haemagglutination antibody titer), and phagocytic activity (carbon clearance and polymorphonuclear (PMN) cell activity). Results: Oral administration of NF, SFNF, and SFNFCUD showed a significant (p<0.01) increase in adhesion of neutrophils, potentiation of the DTH reaction, and attenuation of cyclophosphamide-induced neutropenia. A significant increase in serum immunoglobulin levels and production of circulating antibody titer in response to sheep red blood cells (RBCs) was also observed. In addition, an increase in the phagocytic index in the carbon clearance assay and an increase in the phagocytic activity of PMN cells were observed. Conclusion: From the above results, it can be concluded that all three types of formulations showed significant immunostimulant activity. SFNF and SFNFCUD showed better immunomodulatory activity than NF suggesting the potentiation of immunomodulatory potential of NF activity by fortification methods.